The effects of aging on molecular modulators of human embryo implantation

Panagiotis Ntostis; Grace Swanson; Georgia Kokkali; David Iles; John Huntriss; Agni Pantou; Maria Tzetis; Konstantinos Pantos; Helen M. Picton; Stephen A. Krawetz; David Miller

iScience (Jul 2021)

The effects of aging on molecular modulators of human embryo implantation

Panagiotis Ntostis,
Grace Swanson,
Georgia Kokkali,
David Iles,
John Huntriss,
Agni Pantou,
Maria Tzetis,
Konstantinos Pantos,
Helen M. Picton,
Stephen A. Krawetz,
David Miller

Affiliations

Panagiotis Ntostis: Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK; Genetics Department, Medical school, National and Kapodistrian University of Athens, Athens, 115 27, Greece; Corresponding author
Grace Swanson: Department of Obstetrics and Gynecology and the Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USA
Georgia Kokkali: Genesis Athens Clinic, Reproductive Medicine Unit, Athens, 152 32, Greece
David Iles: Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK
John Huntriss: Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK
Agni Pantou: Genesis Athens Clinic, Reproductive Medicine Unit, Athens, 152 32, Greece
Maria Tzetis: Genetics Department, Medical school, National and Kapodistrian University of Athens, Athens, 115 27, Greece
Konstantinos Pantos: Genesis Athens Clinic, Reproductive Medicine Unit, Athens, 152 32, Greece
Helen M. Picton: Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK
Stephen A. Krawetz: Department of Obstetrics and Gynecology and the Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USA
David Miller: Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK; Corresponding author

Journal volume & issue: Vol. 24, no. 7
p. 102751

Abstract

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Summary: Advancing age has a negative impact on female fertility. As implantation rates decline during the normal maternal life course, age-related, embryonic factors are altered and our inability to monitor these factors in an unbiased genome-wide manner in vivo has severely limited our understanding of early human embryo development and implantation. Our high-throughput methodology uses trophectoderm samples representing the full spectrum of maternal reproductive ages with embryo implantation potential examined in relation to trophectoderm transcriptome dynamics and reproductive maternal age. Potential embryo-endometrial interactions were tested using trophectoderm sampled from young women, with the receptive uterine environment representing the most ‘fertile’ environment for successful embryo implantation. Potential roles for extracellular exosomes, embryonic metabolism and regulation of apoptosis were revealed. These biomarkers are consistent with embryo-endometrial crosstalk/developmental competency, serving as a mediator for successful implantation. Our data opens the door to developing a diagnostic test for predicting implantation success in women undergoing fertility treatment.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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