EFFECT OF NAA, IBA AND MS STRENGTH IN ROOTING OF Bauhinia purpurea L. IN VITRO

Abstract

This study aimed to assess the effect of concentrations 0.0, 0.25, 0.5, 0.75, 1.0, 1.5, 2.0, 2.5, 3.0 mg/l of each of IBA and NAA and MS Strength in shoot rooting of Purple camel's foot the plantlet produced from the buds in multiplication stage on MS with 3.0 mg/l BA or 2.0 mg/l TDZ. Rooting results using full strength MS medium showed that 0.75 mg/l IBA produced largest average of root numbers (15.55). Also the same concentration of IBA have similar effect on lengths of roots (38.88 cm). On the other hand the response of shoots for rooting in different concentration of NAA was high and the 2.0 mg/l concentration of NAA was significant for roots numbers averages (8.44). The MS medium with half strength was tested for rooting with addition same concentrations of IBA and NAA, So 1.5 mgl IBA have highest numbers of roots (9.88) and longest roots trait (25.05 cm.). but 3.0 mg/l NAA was superior in comparison with all the other concentrations and have largest numbers for shoots with recorde (11.66), but 0.25 mgl NAA gave highest roots (15.77). In this study it was possible to acclimatized the plant growing in tubes, plantlets were hardened gradually by removed the plantlets from the tubes washing them thoroughly with water to remove the residual agar from the roots, then planted in pots containing mixture of sand and petmous (1:1). and covered with light permeable plastic sheets with pores to inter air. percentage (80%) was achieved.

Keywords

• this study aimed to assess the effect of concentrations 0.0
• 0.25
• 0.5
• 0.75
• 1.0
• 1.5
• 2.0
• 2.5
• 3.0 mg/l of each of iba and naa and ms strength in shoot rooting of purple camel's foot the plantlet produced from the buds in multiplication stage on ms with 3.0 mg/l ba or 2.0 mg/l tdz. rooting results using full strength ms medium showed that 0.75
• so 1.5 mgl iba have highest numbers of roots (9.88) and longest roots trait (25.05 cm.). but 3.0 mg/l naa was superior in comparison with all the other concentrations and have largest numbers for shoots with recorde (11.66)
• but 0.25 mgl naa gave highest roots (15.77). in this study it was possible to acclimatized the plant growing in tubes
• plantlets were hardened gradually by removed the plantlets from the tubes washing them thoroughly with water to remove the residual agar from the roots
• then planted in pots containing mixture of sand and petmous (1:1). and covered with light permeable plastic sheets with pores to inter air. percentage (80%) was achieved