Identification of polyvalent passive vaccine candidates from egg yolk antibodies (IgY) of important outer membrane proteins of Aeromonas hydrophila in fish

Xiang Liu; Jia Chao; Huihui Xiao; Jing Chen; Pan Cui; Xiaoqing Wu; Juan Lu; Juan Wang; Chunlin Chen; Xiaoying Zhang; Gaoxiao Xu; Yong Liu

Aquaculture Reports (Apr 2024)

Identification of polyvalent passive vaccine candidates from egg yolk antibodies (IgY) of important outer membrane proteins of Aeromonas hydrophila in fish

Xiang Liu,
Jia Chao,
Huihui Xiao,
Jing Chen,
Pan Cui,
Xiaoqing Wu,
Juan Lu,
Juan Wang,
Chunlin Chen,
Xiaoying Zhang,
Gaoxiao Xu,
Yong Liu

Affiliations

Xiang Liu: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang 236041, China; Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China; Fuyang Normal University--Funan Rural Revitalization Collaborative Technology Service Center, Fuyang Normal University, Fuyang 236041, China; Corresponding author at: Fuyang Normal University--Funan Rural Revitalization Collaborative Technology Service Center, Fuyang Normal University, Fuyang 236041, China.
Jia Chao: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang 236041, China; Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China
Huihui Xiao: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang 236041, China; Fuyang Normal University--Funan Rural Revitalization Collaborative Technology Service Center, Fuyang Normal University, Fuyang 236041, China
Jing Chen: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang 236041, China
Pan Cui: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang 236041, China
Xiaoqing Wu: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang 236041, China
Juan Lu: Fuyang Normal University--Funan Rural Revitalization Collaborative Technology Service Center, Fuyang Normal University, Fuyang 236041, China
Juan Wang: Fuyang Normal University--Funan Rural Revitalization Collaborative Technology Service Center, Fuyang Normal University, Fuyang 236041, China
Chunlin Chen: Fuyang Normal University--Funan Rural Revitalization Collaborative Technology Service Center, Fuyang Normal University, Fuyang 236041, China
Xiaoying Zhang: Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China
Gaoxiao Xu: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang 236041, China; Correspondence to: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236041, China.
Yong Liu: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang 236041, China; Fuyang Normal University--Funan Rural Revitalization Collaborative Technology Service Center, Fuyang Normal University, Fuyang 236041, China; Correspondence to: Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Fuyang Normal University, Fuyang 236041, China.

Journal volume & issue: Vol. 35
p. 102002

Abstract

Read online

The antibodies of outer membrane proteins (OMPs) of bacteria have application value in polyvalent passive vaccines. IgY antibodies can be obtained in large quantities and economically from egg yolk, and have the potential to be applied in aquaculture. The OMPs (OmpAII, OmpW, P5, and Slp) of Aeromonas hydrophila were expressed and purified, and the corresponding IgY antibodies were prepared. Passive immunization to goldfish (Carassius auratus) indicated that the four IgY antibodies had an effective immune protection rate, could activate the innate immune responses, down-regulated the expression of inflammation-related genes (p < 0.05) to reduce inflammatory reactions, down-regulated the expression of antioxidant-related factors (p < 0.05) to reduce antioxidant reactions, protected the integrity of visceral tissue structure, and reduced apoptosis and DNA damage for kidney cells infected by A. hydrophila and Pseudomonas fluorescens. Moreover, the IgY passive (A. hydrophila) and passive cross-protective (P. fluorescens) abilities of OmpW and Slp were higher than those of OmpAII and P5. Thus, the IgY antibodies of OmpW and Slp can be considered as polyvalent vaccine candidates for passive immunization in aquaculture.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

About the journal

Abstract

Keywords