Phylogenetic Analysis of H1N1 Proteins for Understanding Its Allocation

Abstract

A serious Influenza pandemic infection has rapidly spread across the world since 2009 causing nearly 300,000 deaths globally within the first year of the pandemic. In 2014 and 2015, the swine flu pandemic hits again with increased rate of reported cases. H1N1, a swine influenza virus is a known causative agent of swine flu. This outbreak caused by subtype H1N1 in humans is due to transfer of swine influenza virus from pig to human. The entire Influenza A virus genome contained eight RNA segments such as Matrix protein, Hemagglutinin, Neuraminidase, Nucleocapsid protein, Polymerase PB1, Polymerase PA, Polymerase PB2 and Non-structural protein. In this study, phylogenetic analysis of protein sequences of human H1N1 viruses was carried out using MEGA 6 software to demonstrate the route map of its infection. Phylogenetic analysis of the sequences from Asian and other foreign countries, available at NCBI protein database were retrieved and analyzed. The result shows that many proteins of Indian entries were clustered within India while few with close Asian and foreign countries and very few remained non-clustered as evidenced in all sequences. The study helps understood the distribution and flow pattern of H1N1 virus across the world and Phylogenetic tools predicted the defined phyletic clusters available in that population.

Keywords

• Swine flu
• H1N1
• Influenza
• Phylogenetic analysis