Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential

Duan Jinliang; Bai Fang; Zhu Xiaofeng; He Xiaoshun; Hu Anbin

Stem Cell Research (Mar 2024)

Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential

Duan Jinliang,
Bai Fang,
Zhu Xiaofeng,
He Xiaoshun,
Hu Anbin

Affiliations

Duan Jinliang: Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong,China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, Guangdong, China
Bai Fang: Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong,China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, Guangdong, China
Zhu Xiaofeng: Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong,China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, Guangdong, China
He Xiaoshun: Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong,China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, Guangdong, China
Hu Anbin: Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong,China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial International Cooperation Base of Science and Technology(Organ Transplantation), Guangzhou, Guangdong, China; Corresponding author.

Journal volume & issue: Vol. 75
p. 103302

Abstract

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CCL22 is a macrophage-derived immunosuppressive chemokine that recruits regulatory T cells through the CCL22:CCR4 axis, playing an important role in homeostatic and inflammatory responses. A CCL22-overexpressing human induced pluripotent stem cell line (CNNDi001-A-2) was generated by lentiviral transduction to further study the function of CCL22. The cell line was confirmed to have normal proliferation and pluripotency and could be further differentiated into islet cells for cell replacement therapy in diabetes.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal