Some biochemical constituents in follicular fluid of indigenous cows of Assam

Abstract

Aim: Estimation of some biochemical constituents in follicular fluid of non-descript cows of Assam. Materials and Methods: Twenty five pairs of ovaries were collected from local slaughter house and the follicular fluid was aspirated from small (2-4 mm), medium (4-6 mm) and large (6-9 mm) follicles. Aspirated fluid samples were centrifuged at 4000 rpm for 20 min in a refrigerated centrifuge to remove granulosa cells and other cell debris. Supernatant was used for estimation of glucose, total protein, cholesterol, acid phosphatase and alkaline phosphatase. Data generated in the study were analyzed statiscally by SPSS (version 16.0). SPSS South Asia Pvt. Limited, Kacharakanahalli, Bangalore, 560043). Results: A non significant difference was recorded in case of total protein and cholesterol of follicular fluid of small, medium and large sized follicles of cow. However, the glucose level significantly (p<0.01) increased with the increase of follicular size where as the activity of acid phosphatase and alkaline phosphatase significantly (p<0.01) decreased with increase of size. Conclusion: Certain biochemical constituents and enzyme activities of follicular fluid changes with the growth of follicles in non-descript cows of Assam. The glucose concentration increased with the growth of the follicles while acid phosphatase and alkaline phosphatase levels had an inverse relation with the size of the ovarian follicles.

Keywords

• biochemical parameters
• follicles
• follicular fluid
• oocytes maturation
• phosphatase