精准医学杂志 (Feb 2023)
EXPRESSION AND SIGNIFICANCE OF LONG NON-CODING RNAS AND MICRORNAS IN EPITHELIAL-MESENCHYMAL TRANSITION MODEL OF HUMAN BRONCHIAL EPITHELIAL CELLS CONSTRUCTED BY TRANSFORMING GROWTH FACTOR-BETA 1 INTERVENTION
Abstract
Objective To investigate the expression of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in the epithelial-mesenchymal transition (EMT) model of human bronchial epithelial cell line 16HBE induced by human transforming growth factor-β1 (TGF-β1). Methods 16HBE cells were cultured in vitro, and the experiment established control group and treatment group. The cells in the treatment group were induced by TGF-β1 to establish an EMT cell model, and those in the control group were added with an equal volume of RPMI basic culture medium. At 24, 48, and 72 h after TGF-β1 treatment, an inverted microscope was used to observe cell morphology and distribution. At the time point when the cells in the treatment group began to show obvious spindle-shaped changes, the methods of RNA separation, reverse transcription, and quantitative real-time PCR were used to measure the relative expression levels of lncRNAs and miRNAs in the two groups. Results After 48 h of TGF-β1 treatment, the cells in the treatment group showed obvious spindle-shaped changes and an increase in intercellular space, suggesting that the EMT model was successfully established. At this time point, there were significant differences between the two groups in the relative expression levels of TBILA, NKILA, LNCRNA-ATB, HOTAIR, NEAT1, miR-125b-5p, miR-21-3p, miR-21-5p, miR-27a-3p, miR-27a-5p, miR-141-3p, miR-200c-3p, miR-17-5p, miR-34a-5p(t=-16.353-6.460,P<0.05). Conclusion TGF-β1 intervention can successfully establish the EMT cell model, and the differentially expressed lncRNAs and miRNAs between this cell model and normal cells may be involved in the formation and development of EMT or airway remodeling, which might provide a theoretical basis for the prevention and treatment of such diseases.
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