Cross-linking N-succinyl chitosan-oxidated hyaluronic acid-based hydrogel loaded with bone marrow mesenchymal stem cell-derived exosomes induce bone regeneration in cranial defects

Xiao-yang Chu; Kai Yang; Xin He; Kai-tao Yu; Ying-yi Luan; Qing-bing He; Ze-lu Li; Yu-lan Xiang; Huaiwen Chen; Yang Zeng; Ya-Zhou Li; Dong-liang Zhang

Materials & Design (May 2024)

Cross-linking N-succinyl chitosan-oxidated hyaluronic acid-based hydrogel loaded with bone marrow mesenchymal stem cell-derived exosomes induce bone regeneration in cranial defects

Xiao-yang Chu,
Kai Yang,
Xin He,
Kai-tao Yu,
Ying-yi Luan,
Qing-bing He,
Ze-lu Li,
Yu-lan Xiang,
Huaiwen Chen,
Yang Zeng,
Ya-Zhou Li,
Dong-liang Zhang

Affiliations

Xiao-yang Chu: Department of Stomatology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
Kai Yang: Prenatal Diagnosis Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Healthcare Hospital, Beijing, China
Xin He: Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, Beijing, China
Kai-tao Yu: Department of Stomatology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China
Ying-yi Luan: Prenatal Diagnosis Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Healthcare Hospital, Beijing, China
Qing-bing He: Department of Pediatric Orthopaedics, the Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, China
Ze-lu Li: Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, Beijing, China
Yu-lan Xiang: Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, Beijing, China
Huaiwen Chen: Sunlipo Biotech Research Center for Nanomedicine, Shanghai, China
Yang Zeng: State Key Laboratory of Experimental Hematology, Haihe Laboratory of Cell Ecosystem, Senior Department of Hematology, Fifth Medical Center, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing, China; Corresponding authors at: Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, 11 Xilahutong Street (Pewter Lane), Beijing 100006, China (D. Zhang); Department of Pediatric Orthopedics, the Third Hospital of Hebei Medical University, 139 Ziqiang Road, Shijiazhuang, Hebei 050051, China (Y.-z. Li); State Key Laboratory of Experimental Hematology, Haihe Laboratory of Cell Ecosystem, Senior Department of Hematology, Fifth Medical Center, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing 100071, China (Y. Zeng).
Ya-Zhou Li: Department of Pediatric Orthopaedics, the Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, China; Corresponding authors at: Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, 11 Xilahutong Street (Pewter Lane), Beijing 100006, China (D. Zhang); Department of Pediatric Orthopedics, the Third Hospital of Hebei Medical University, 139 Ziqiang Road, Shijiazhuang, Hebei 050051, China (Y.-z. Li); State Key Laboratory of Experimental Hematology, Haihe Laboratory of Cell Ecosystem, Senior Department of Hematology, Fifth Medical Center, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing 100071, China (Y. Zeng).
Dong-liang Zhang: Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, Beijing, China; Corresponding authors at: Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University School of Stomatology, Capital Medical University, 11 Xilahutong Street (Pewter Lane), Beijing 100006, China (D. Zhang); Department of Pediatric Orthopedics, the Third Hospital of Hebei Medical University, 139 Ziqiang Road, Shijiazhuang, Hebei 050051, China (Y.-z. Li); State Key Laboratory of Experimental Hematology, Haihe Laboratory of Cell Ecosystem, Senior Department of Hematology, Fifth Medical Center, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing 100071, China (Y. Zeng).

Journal volume & issue: Vol. 241
p. 112969

Abstract

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Background: Hydrogel carrying exosomes shows better prospects for bone regeneration. Objective: This work investigated the effect of cross-linking N-succinyl chitosan-oxidated hyaluronic acid-based hydrogel loaded with bone marrow mesenchymal stem cell-derived exosomes on bone regeneration in cranial defects. Methods: Cross-linking N-succinyl chitosan-oxidated hyaluronic acid-based hydrogel loaded with bone marrow mesenchymal stem cell-derived exosomes was prepared, and utilized to treat bone marrow mesenchymal stem cells, human umbilical vein endothelial cells, and rats with cranial defects. Logical experiments were implemented to research the effect of the prepared hydrogel on osteogenesis and angiogenesis of cells in vitro, and on bone regeneration in rats with cranial defects. Results: Cross-linking N-succinyl chitosan-oxidated hyaluronic acid-based hydrogel loaded with bone marrow mesenchymal stem cell-derived exosomes showed good biocompatibility and self-healing property. It promoted osteogenic differentiation of bone marrow mesenchymal stem cells and angiogenesis of human umbilical vein endothelial cells, and facilitated osteogenic differentiation, angiogenesis and bone regeneration in rats with cranial defects. Conclusion: Cross-linking N-succinyl chitosan-oxidated hyaluronic acid-based hydrogel loaded with bone marrow mesenchymal stem cell-derived exosomes facilitated osteogenesis, angiogenesis and bone regeneration in rats with cranial defects. It might be an effective strategy for inducing bone regeneration in individuals with cranial defects.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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