Generation of Integration-free and Region-Specific Neural Progenitors from Primate Fibroblasts

Jianfeng Lu; Huisheng Liu; Cindy Tzu-Ling Huang; Hong Chen; Zhongwei Du; Yan Liu; Mohammad Amin Sherafat; Su-Chun Zhang

doi:10.1016/j.celrep.2013.04.004

Cell Reports (May 2013)

Generation of Integration-free and Region-Specific Neural Progenitors from Primate Fibroblasts

Jianfeng Lu,
Huisheng Liu,
Cindy Tzu-Ling Huang,
Hong Chen,
Zhongwei Du,
Yan Liu,
Mohammad Amin Sherafat,
Su-Chun Zhang

Affiliations

Jianfeng Lu: Departments of Neuroscience and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, Madison, WI 53705, USA
Huisheng Liu: Departments of Neuroscience and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, Madison, WI 53705, USA
Cindy Tzu-Ling Huang: Departments of Neuroscience and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, Madison, WI 53705, USA
Hong Chen: Departments of Neuroscience and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, Madison, WI 53705, USA
Zhongwei Du: Departments of Neuroscience and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, Madison, WI 53705, USA
Yan Liu: Departments of Neuroscience and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, Madison, WI 53705, USA
Mohammad Amin Sherafat: Departments of Neuroscience and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, Madison, WI 53705, USA
Su-Chun Zhang: Departments of Neuroscience and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, Madison, WI 53705, USA

DOI: https://doi.org/10.1016/j.celrep.2013.04.004
Journal volume & issue: Vol. 3, no. 5
pp. 1580 – 1591

Abstract

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Postnatal and adult human and monkey fibroblasts were infected with Sendai virus containing the Yamanaka factors for 24 hr, then they were cultured in a chemically defined medium containing leukemia inhibitory factor (LIF), transforming growth factor (TGF)-β inhibitor SB431542, and glycogen synthase kinase (GSK)-3β inhibitor CHIR99021 at 39°C for inactivation of the virus. Induced neural progenitor (iNP) colonies appeared as early as day 13 and can be expanded for >20 passages. Under the same defined condition, no induced pluripotent stem cell (iPSC) colonies formed at either 37°C or 39°C. The iNPs predominantly express hindbrain genes and differentiate into hindbrain neurons, and when caudalized, they produced an enriched population of spinal motor neurons. Following transplantation into the forebrain, the iNP-derived cells retained the hindbrain identity. The ability to generate defined, integration-free iNPs from adult primate fibroblasts under a defined condition with predictable fate choices will facilitate disease modeling and therapeutic development.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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