Spatial segregation of Darwinula stevensoni (Crustacea: Ostracoda) genotypes in lentic and lotic habitats of Northern Italy

Abstract

The clonal structure of apomictic populations of Darwinula stevensoni from Northern Italy lacustrine and riverine habitats has been analysed by allozyme electrophoresis. Thirty two sites were sampled and a total of 748 individuals were analysed. Only one polymorphic locus, Gpi, turned out to have enough variation to be informative. Two thirds of all individuals were homozygous for the most frequent allele but the frequency of this genotype was significantly lower in rivers (16%) than in lakes (92%). As a consequence homozygous clonal females are dominant in lacustrine habitats while heterozygous clonal females are dominant in riverine ones. Differences in genetic structure between habitats were observed even at distances of a few meters. Allele and genotype frequencies are significantly different between Lake Montorfano and its outflow (River Seveso) and between Mantova lakes and their two tributaries, the River Mincio and Corniano Channel. The former is also the outflow of Lake Garda. By laboratory experiments, we previously reported difference between genotypes: homozygous females from Mantova lakes show significantly lower starvation tolerance than heterozygous females from River Mincio. We discuss the hypothesis that habitat segregation is related to this difference. Riverine females should be better adapted to low productivity ecosystems than lacustrine ones.

Keywords

• apomictic parthenogenesis, clonal diversity, habitat specialisation