Crop Breeding and Applied Biotechnology (Jan 2009)

Validation of microsatellite markers for assisted selection of soybean genotypes resistant to powdery mildew

  • Paula dos Santos Demore,
  • Sandra Helena Unêda-Trevisoli,
  • Antonio Orlando Di Mauro,
  • Thaiza Galhardo Silva Morceli,
  • Franco Romero Silva Muniz,
  • Marcelo Marchi Costa,
  • Daniela Garcia Penha Sarti,
  • Melina Cristina Mancini

Journal volume & issue
Vol. 9, no. 1
pp. 45 – 51

Abstract

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Powdery mildew is one of the most serious diseases of soybean and is found in all producing countries. Thepurpose of this study was to validate microsatellite markers previously identified as associated with resistance to powderymildew in soybean. The study was conducted in two F2 parent populations with contrasting resistance to powdery mildew. Inthe analysis 10 SSR primers were used for the populations, and four polymorphic markers were identified for cross 1(MGBR95-20937 x IAC-Foscarin 31) and three for cross 2 (MGBR-46 x EMBRAPA 48). The Chi-square analysis of thephenotypic evaluation confirmed the expected segregation (3:1) of a dominant gene related to resistance. The polymorphicmarkers also segregated as expected (1:2:1). The markers Sat_366 and Sat_393 in the crosses 1 and 2, respectively, locatedat 9.41 and 12.45 cM from the gene, were considered promising for marker-assisted selection for resistance to powderymildew in soybean, at a selection efficiency of 92.7% and 60.3% respectively.

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