Journal of Fungi (Jul 2024)
Genomic Analysis of <i>Aspergillus</i> Section <i>Terrei</i> Reveals a High Potential in Secondary Metabolite Production and Plant Biomass Degradation
- Sebastian Theobald,
- Tammi C. Vesth,
- Elena Geib,
- Jane L. Nybo,
- Jens C. Frisvad,
- Thomas O. Larsen,
- Alan Kuo,
- Kurt LaButti,
- Ellen K. Lyhne,
- Inge Kjærbølling,
- Line Ledsgaard,
- Kerrie Barry,
- Alicia Clum,
- Cindy Chen,
- Matt Nolan,
- Laura Sandor,
- Anna Lipzen,
- Stephen Mondo,
- Jasmyn Pangilinan,
- Asaf Salamov,
- Robert Riley,
- Ad Wiebenga,
- Astrid Müller,
- Roland S. Kun,
- Ana Carolina dos Santos Gomes,
- Bernard Henrissat,
- Jon K. Magnuson,
- Blake A. Simmons,
- Miia R. Mäkelä,
- Uffe H. Mortensen,
- Igor V. Grigoriev,
- Matthias Brock,
- Scott E. Baker,
- Ronald P. de Vries,
- Mikael R. Andersen
Affiliations
- Sebastian Theobald
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Tammi C. Vesth
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Elena Geib
- School of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK
- Jane L. Nybo
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Jens C. Frisvad
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Thomas O. Larsen
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Alan Kuo
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Kurt LaButti
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Ellen K. Lyhne
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Inge Kjærbølling
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Line Ledsgaard
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Kerrie Barry
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Alicia Clum
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Cindy Chen
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Matt Nolan
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Laura Sandor
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Anna Lipzen
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Stephen Mondo
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Jasmyn Pangilinan
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Asaf Salamov
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Robert Riley
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Ad Wiebenga
- Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, 3584 Utrecht, The Netherlands
- Astrid Müller
- Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, 3584 Utrecht, The Netherlands
- Roland S. Kun
- Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, 3584 Utrecht, The Netherlands
- Ana Carolina dos Santos Gomes
- Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, 3584 Utrecht, The Netherlands
- Bernard Henrissat
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Jon K. Magnuson
- Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA
- Blake A. Simmons
- Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA
- Miia R. Mäkelä
- Department of Microbiology, University of Helsinki, Viikinkaari 9, 00014 Helsinki, Finland
- Uffe H. Mortensen
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- Igor V. Grigoriev
- US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
- Matthias Brock
- School of Life Sciences, University of Nottingham, Nottingham NG7 2RD, UK
- Scott E. Baker
- Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA
- Ronald P. de Vries
- Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, 3584 Utrecht, The Netherlands
- Mikael R. Andersen
- Department of Biotechnology and Bioengineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
- DOI
- https://doi.org/10.3390/jof10070507
- Journal volume & issue
-
Vol. 10,
no. 7
p. 507
Abstract
Aspergillus terreus has attracted interest due to its application in industrial biotechnology, particularly for the production of itaconic acid and bioactive secondary metabolites. As related species also seem to possess a prosperous secondary metabolism, they are of high interest for genome mining and exploitation. Here, we present draft genome sequences for six species from Aspergillus section Terrei and one species from Aspergillus section Nidulantes. Whole-genome phylogeny confirmed that section Terrei is monophyletic. Genome analyses identified between 70 and 108 key secondary metabolism genes in each of the genomes of section Terrei, the highest rate found in the genus Aspergillus so far. The respective enzymes fall into 167 distinct families with most of them corresponding to potentially unique compounds or compound families. Moreover, 53% of the families were only found in a single species, which supports the suitability of species from section Terrei for further genome mining. Intriguingly, this analysis, combined with heterologous gene expression and metabolite identification, suggested that species from section Terrei use a strategy for UV protection different to other species from the genus Aspergillus. Section Terrei contains a complete plant polysaccharide degrading potential and an even higher cellulolytic potential than other Aspergilli, possibly facilitating additional applications for these species in biotechnology.
Keywords
- genomics
- fungi
- <i>Aspergillus</i>
- section <i>Terrei</i>
- <i>Aspergillus terreus</i>
- secondary metabolism
