Role of Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 during Competitive Colonization Interaction against Pectobacterium aroidearum SM2

Mayra Paola Mena Navarro; Merle Ariadna Espinosa Bernal; Adriana Eunice Martinez-Avila; Leonela Sofia Aponte Pineda; Luis Alberto Montes Flores; Carlos Daniel Chan Ku; Yoali Fernanda Hernández Gómez; Jacqueline González Espinosa; Juan Ramiro Pacheco Aguilar; Miguel Ángel Ramos López; Jackeline Lizzeta Arvizu Gómez; Carlos Saldaña Gutierrez; José Alberto Rodríguez Morales; Aldo Amaro Reyes; José Luis Hernández Flores; Juan Campos Guillén

doi:10.3390/microorganisms12050930

Microorganisms (May 2024)

Role of Volatile Organic Compounds Produced by Kosakonia cowanii Cp1 during Competitive Colonization Interaction against Pectobacterium aroidearum SM2

Mayra Paola Mena Navarro,
Merle Ariadna Espinosa Bernal,
Adriana Eunice Martinez-Avila,
Leonela Sofia Aponte Pineda,
Luis Alberto Montes Flores,
Carlos Daniel Chan Ku,
Yoali Fernanda Hernández Gómez,
Jacqueline González Espinosa,
Juan Ramiro Pacheco Aguilar,
Miguel Ángel Ramos López,
Jackeline Lizzeta Arvizu Gómez,
Carlos Saldaña Gutierrez,
José Alberto Rodríguez Morales,
Aldo Amaro Reyes,
José Luis Hernández Flores,
Juan Campos Guillén

Affiliations

Mayra Paola Mena Navarro: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Merle Ariadna Espinosa Bernal: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Adriana Eunice Martinez-Avila: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Leonela Sofia Aponte Pineda: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Luis Alberto Montes Flores: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Carlos Daniel Chan Ku: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Yoali Fernanda Hernández Gómez: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Jacqueline González Espinosa: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Juan Ramiro Pacheco Aguilar: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Miguel Ángel Ramos López: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
Jackeline Lizzeta Arvizu Gómez: Secretaría de Investigación y Posgrado, Centro Nayarita de Innovación y Transferencia de Tecnología (CENITT), Universidad Autónoma de Nayarit, Tepic 63173, Mexico
Carlos Saldaña Gutierrez: Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av. De las Ciencias s/n, Querétaro 76220, Mexico
José Alberto Rodríguez Morales: Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Mexico
Aldo Amaro Reyes: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico
José Luis Hernández Flores: Centro de Investigación y de Estudios Avanzados del IPN, Irapuato 36824, Mexico
Juan Campos Guillén: Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Querétaro 76010, Qro, Mexico

DOI: https://doi.org/10.3390/microorganisms12050930
Journal volume & issue: Vol. 12, no. 5
p. 930

Abstract

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The competitive colonization of bacteria on similar ecological niches has a significant impact during their establishment. The synthesis speeds of different chemical classes of molecules during early competitive colonization can reduce the number of competitors through metabolic effects. In this work, we demonstrate for the first time that Kosakonia cowanii Cp1 previously isolated from the seeds of Capsicum pubescens R. P. produced volatile organic compounds (VOCs) during competitive colonization against Pectobacterium aroidearum SM2, affecting soft rot symptoms in serrano chili (Capsicum annuum L.). The pathogen P. aroidearum SM2 was isolated from the fruits of C. annuum var. Serrano with soft rot symptoms. The genome of the SM2 strain carries a 5,037,920 bp chromosome with 51.46% G + C content and 4925 predicted protein-coding genes. It presents 12 genes encoding plant-cell-wall-degrading enzymes (PCDEWs), 139 genes involved in five types of secretion systems, and 16 genes related to invasion motility. Pathogenic essays showed soft rot symptoms in the fruits of C. annuum L., Solanum lycopersicum, and Physalis philadelphica and the tubers of Solanum tuberosum. During the growth phases of K. cowanii Cp1, a mix of VOCs was identified by means of HS-SPME-GC-MS. Of these compounds, 2,5-dimethyl-pyrazine showed bactericidal effects and synergy with acetoin during the competitive colonization of K. cowanii Cp1 to completely reduce soft rot symptoms. This work provides novel evidence grounding a better understanding of bacterial interactions during competitive colonization on plant tissue, where VOC synthesis is essential and has a high potential capacity to control pathogenic microorganisms in agricultural systems.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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