Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement

Abstract

DNA2 encodes a 5′ flap DNA endonuclease involved in replication and DNA double strand break processing. Here the authors by using a conditional degron system together with electron microscopy characterize the role played by Dna2 and Pif1 helicase during unperturbed DNA replication in S. cerevisiae.