The interdomain flexible linker of the polypeptide GalNAc transferases dictates their long-range glycosylation preferences

Matilde de las Rivas; Erandi Lira-Navarrete; Earnest James Paul Daniel; Ismael Compañón; Helena Coelho; Ana Diniz; Jesús Jiménez-Barbero; Jesús M. Peregrina; Henrik Clausen; Francisco Corzana; Filipa Marcelo; Gonzalo Jiménez-Osés; Thomas A. Gerken; Ramon Hurtado-Guerrero

doi:10.1038/s41467-017-02006-0

Nature Communications (Dec 2017)

The interdomain flexible linker of the polypeptide GalNAc transferases dictates their long-range glycosylation preferences

Matilde de las Rivas,
Erandi Lira-Navarrete,
Earnest James Paul Daniel,
Ismael Compañón,
Helena Coelho,
Ana Diniz,
Jesús Jiménez-Barbero,
Jesús M. Peregrina,
Henrik Clausen,
Francisco Corzana,
Filipa Marcelo,
Gonzalo Jiménez-Osés,
Thomas A. Gerken,
Ramon Hurtado-Guerrero

Affiliations

Matilde de las Rivas: BIFI, University of Zaragoza, BIFI-IQFR (CSIC) Joint Unit, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D
Erandi Lira-Navarrete: BIFI, University of Zaragoza, BIFI-IQFR (CSIC) Joint Unit, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D
Earnest James Paul Daniel: Department of Biochemistry, Case Western Reserve University
Ismael Compañón: Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química
Helena Coelho: UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Nova de Lisboa
Ana Diniz: UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Nova de Lisboa
Jesús Jiménez-Barbero: CIC bioGUNE, Bizkaia Technology Park
Jesús M. Peregrina: Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química
Henrik Clausen: Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, School of Dentistry, University of Copenhagen
Francisco Corzana: Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química
Filipa Marcelo: UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Nova de Lisboa
Gonzalo Jiménez-Osés: Departamento de Química, Universidad de La Rioja, Centro de Investigación en Síntesis Química
Thomas A. Gerken: Department of Biochemistry, Case Western Reserve University
Ramon Hurtado-Guerrero: BIFI, University of Zaragoza, BIFI-IQFR (CSIC) Joint Unit, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D

DOI: https://doi.org/10.1038/s41467-017-02006-0
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 11

Abstract

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GalNAc transferases’ (GalNAc-Ts) catalytic domains are connected to a lectin domain through a flexible linker. Here the authors present a structural analysis of GalNAc-T4 that implicates the linker region as modulator of the orientations of the lectin domain, which in turn imparts substrate specificity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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