Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patients

Abstract

Protease-resistant protein and lipid mycobacterial antigens are present in biological fluids of pulmonary tuberculosis patients. Anti-tuberculosis monoclonal antibodies (MAb) reacting to proteins participating in the molybdopterin synthesis have been obtained. With the help of two MAb recognizing different epitopes on one antigen with molecular mass of 32, 50 and 75 kilodalton. With the help of which the two-site sandwich of IFA type capable of detecting the mycobacterial antigen in antigenic preparations of х M. tuberculosis and in urine samples of tuberculosis patients. However the specificity of this test is fairly low since when testing urine samples this antigen was found in the half of healthy donors. It is due to the fact that in human urogenital tract some opportunistic pathogens are present which belong to normal bacterial population with which the tested MAb have the cross-reaction.

Keywords

• микобактериальные антигены
• моноклональные антитела
• гибридомная технология
• гибридомная технология