Di-san junyi daxue xuebao (Jan 2022)
Effect of pinoresinol diglucoside on bone quality in growing rats
Abstract
Objective To determine the effect of pinoresinol diglucoside (PDG) on bone quality in growing rats and evaluate its potential as an anti-osteoporosis drug. Methods A total of 30 1-month-old female SD rats (body weight 107±7 g) were randomly divided into 3 groups: control group (Control), 25 mg/(kg·d) PDG treatment group (PDG-25) and 50 mg/(kg·d) PDG treatment group (PDG-50), with 10 rats in each group. The rats in the PDG-25 group and PDG-50 group were given corresponding doses of PDG intragastrically every day, and those of the control group was given equal volume distilled water. Their body mineral density (BMD) of the whole body was measured in 2, 4 and 6 weeks after treatment. In the end of 6 weeks, all rats were sacrificed, and organ coefficients of important organs were calculated. Micro-CT scanning, bone biomechanical test and serum biochemical tests of bone metabolism were performed. Results There were no significant differences in body weight and main organ coefficients among the groups. At 6 weeks, the BMD value was significantly higher in the PDG-25 group and PDG-50 group than the Control group (P < 0.05). The PDG-50 group had significantly higher relative bone volume, trabecular number and volumetric bone mineral density (P < 0.05), lower trabecular separation (P < 0.05) than the Control group, and the bone microstructure of PDG-25 group and PDG-50 group was significantly better than that of Control group. The PDG-50 group had elevated elastic modulus of femur (P < 0.05), larger maximum load of vertebrae (P < 0.05). The PDG-50 group had increased content of serum osteocalcin (P < 0.01), but obviously lower content of serum tartrate resistant acid phosphatase 5b (P < 0.01), when compared with the Control group. The content of serum osteocalcin in the PDG-25 group was significantly higher than that in the Control group (P < 0.05). Conclusion PDG can effectively improve bone mineral density, optimize bone microstructure, enhance bone strength, promote bone formation, inhibit bone resorption and improve bone quality.
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