Effect of Tris and Biociphos-Plus Extenders on the Extracellular Enzyme Release of Phosphatases and Transferases in Punganur Bull Semen

Abstract

The present investigation was carried out to study phosphatase and transferase enzymes leakage in the neat and extended Punganur bull semen before and after freezing with Tris and Biociphos-plus extenders. Among the bulls studied, the Alkaline Phosphatase (AKP) concentration in fresh semen was significantly different but Acid Phosphatase (ACP) was not significantly different and a positive correlation between the concentration of AKP and semen volume, individual motility, sperm concentration and total abnormalities were observed. Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) concentrations were not significantly different and positively correlated with individual motility, sperm concentration, percentage of live sperms and acrosomal damage. Leakage of AKP, ACP, AST and ALT enzymes before freezing the semen of Punganur bulls was observed to be significantly higher in the semen extended with Biociphos-plus extender.