High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange

Sarah A. Overall; Jugmohit S. Toor; Stephanie Hao; Mark Yarmarkovich; Sara M. O’Rourke; Giora I. Morozov; Son Nguyen; Alberto Sada Japp; Nicolas Gonzalez; Danai Moschidi; Michael R. Betts; John M. Maris; Peter Smibert; Nikolaos G. Sgourakis

doi:10.1038/s41467-020-15710-1

Nature Communications (Apr 2020)

High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange

Sarah A. Overall,
Jugmohit S. Toor,
Stephanie Hao,
Mark Yarmarkovich,
Sara M. O’Rourke,
Giora I. Morozov,
Son Nguyen,
Alberto Sada Japp,
Nicolas Gonzalez,
Danai Moschidi,
Michael R. Betts,
John M. Maris,
Peter Smibert,
Nikolaos G. Sgourakis

Affiliations

Sarah A. Overall: Department of Chemistry and Biochemistry, University of California Santa Cruz
Jugmohit S. Toor: Department of Chemistry and Biochemistry, University of California Santa Cruz
Stephanie Hao: Technology Innovation Lab, New York Genome Center
Mark Yarmarkovich: Division of Oncology, Center for Childhood Cancer Research, Children’s Hospital of Philadelphia and Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania
Sara M. O’Rourke: Department of Chemistry and Biochemistry, University of California Santa Cruz
Giora I. Morozov: Department of Chemistry and Biochemistry, University of California Santa Cruz
Son Nguyen: Department of Microbiology, Perelman School of Medicine, University of Pennsylvania
Alberto Sada Japp: Department of Microbiology, Perelman School of Medicine, University of Pennsylvania
Nicolas Gonzalez: Department of Chemistry and Biochemistry, University of California Santa Cruz
Danai Moschidi: Department of Chemistry and Biochemistry, University of California Santa Cruz
Michael R. Betts: Department of Microbiology, Perelman School of Medicine, University of Pennsylvania
John M. Maris: Division of Oncology, Center for Childhood Cancer Research, Children’s Hospital of Philadelphia and Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania
Peter Smibert: Technology Innovation Lab, New York Genome Center
Nikolaos G. Sgourakis: Department of Chemistry and Biochemistry, University of California Santa Cruz

DOI: https://doi.org/10.1038/s41467-020-15710-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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Peptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell repertoire/antigen specificity and transcriptome.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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