Accurate detection and quantification of SARS-CoV-2 genomic and subgenomic mRNAs by ddPCR and meta-transcriptomics analysis

Annarita Oranger; Caterina Manzari; Matteo Chiara; Elisabetta Notario; Bruno Fosso; Antonio Parisi; Angelica Bianco; Michela Iacobellis; Morena d’Avenia; Anna Maria D’Erchia; Graziano Pesole

doi:10.1038/s42003-021-02748-0

Communications Biology (Oct 2021)

Accurate detection and quantification of SARS-CoV-2 genomic and subgenomic mRNAs by ddPCR and meta-transcriptomics analysis

Annarita Oranger,
Caterina Manzari,
Matteo Chiara,
Elisabetta Notario,
Bruno Fosso,
Antonio Parisi,
Angelica Bianco,
Michela Iacobellis,
Morena d’Avenia,
Anna Maria D’Erchia,
Graziano Pesole

Affiliations

Annarita Oranger: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro
Caterina Manzari: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro
Matteo Chiara: Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council
Elisabetta Notario: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro
Bruno Fosso: Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council
Antonio Parisi: Istituto Zooprofilattico Sperimentale della Puglia e Basilicata
Angelica Bianco: Istituto Zooprofilattico Sperimentale della Puglia e Basilicata
Michela Iacobellis: Servizio Centralizzato Aziendale di Citopatologia e Screening- PO “Di Venere” - ASL
Morena d’Avenia: Servizio Centralizzato Aziendale di Citopatologia e Screening- PO “Di Venere” - ASL
Anna Maria D’Erchia: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro
Graziano Pesole: Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari Aldo Moro

DOI: https://doi.org/10.1038/s42003-021-02748-0
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 10

Abstract

Read online

Oranger et al use digital droplet PCR to develop an assay that provides a dynamic snapshot of the levels of SARS-CoV-2 sub-genomic messanger RNA in infected individuals. This has the potential to improve diagnostic accuracy for COVID-19

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal