Frontiers in Genetics (Nov 2022)
The expression of apoptosis related genes in HK-2 cells overexpressing PPM1K was determined by RNA-seq analysis
- Li Zhang,
- Li Zhang,
- Li Zhang,
- Li Zhang,
- Li Zhang,
- Xiaohong Sang,
- Xiaohong Sang,
- Xiaohong Sang,
- Xiaohong Sang,
- Xiaohong Sang,
- Yuanyuan Han,
- Yuanyuan Han,
- Yuanyuan Han,
- Yuanyuan Han,
- Yuanyuan Han,
- Alpati Abulitibu,
- Alpati Abulitibu,
- Alpati Abulitibu,
- Alpati Abulitibu,
- Alpati Abulitibu,
- Mufunayi Elken,
- Mufunayi Elken,
- Mufunayi Elken,
- Mufunayi Elken,
- Mufunayi Elken,
- Zhijie Mao,
- Zhijie Mao,
- Zhijie Mao,
- Zhijie Mao,
- Zhijie Mao,
- Shaotao Kang,
- Shaotao Kang,
- Shaotao Kang,
- Shaotao Kang,
- Shaotao Kang,
- Wenjun Yang,
- Wenjun Yang,
- Wenjun Yang,
- Wenjun Yang,
- Wenjun Yang,
- Chen Lu,
- Chen Lu,
- Chen Lu,
- Chen Lu,
- Chen Lu
Affiliations
- Li Zhang
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Li Zhang
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Li Zhang
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Li Zhang
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Li Zhang
- Institute of Nephrology of Xinjiang, Urumqi, China
- Xiaohong Sang
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Xiaohong Sang
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Xiaohong Sang
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Xiaohong Sang
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Xiaohong Sang
- Institute of Nephrology of Xinjiang, Urumqi, China
- Yuanyuan Han
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Yuanyuan Han
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Yuanyuan Han
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Yuanyuan Han
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Yuanyuan Han
- Institute of Nephrology of Xinjiang, Urumqi, China
- Alpati Abulitibu
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Alpati Abulitibu
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Alpati Abulitibu
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Alpati Abulitibu
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Alpati Abulitibu
- Institute of Nephrology of Xinjiang, Urumqi, China
- Mufunayi Elken
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Mufunayi Elken
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Mufunayi Elken
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Mufunayi Elken
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Mufunayi Elken
- Institute of Nephrology of Xinjiang, Urumqi, China
- Zhijie Mao
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Zhijie Mao
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Zhijie Mao
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Zhijie Mao
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Zhijie Mao
- Institute of Nephrology of Xinjiang, Urumqi, China
- Shaotao Kang
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Shaotao Kang
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Shaotao Kang
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Shaotao Kang
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Shaotao Kang
- Institute of Nephrology of Xinjiang, Urumqi, China
- Wenjun Yang
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Wenjun Yang
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Wenjun Yang
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Wenjun Yang
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Wenjun Yang
- Institute of Nephrology of Xinjiang, Urumqi, China
- Chen Lu
- Nephrology Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi, China
- Chen Lu
- Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi, China
- Chen Lu
- Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi, China
- Chen Lu
- Xinjiang Blood Purification Medical Quality Control Center, Urumqi, China
- Chen Lu
- Institute of Nephrology of Xinjiang, Urumqi, China
- DOI
- https://doi.org/10.3389/fgene.2022.1004610
- Journal volume & issue
-
Vol. 13
Abstract
Chronic kidney disease (CKD) is a serious disease that endangers human health. It is reported that inhibiting renal cell apoptosis can delay the progress of CKD. Our previous study found that the mice with protein phosphatase Mg2+/Mn2+ dependent 1K (PPM1K) gene deletion had obvious symptoms of glomerular vascular and interstitial vascular dilatation, congestion and hemorrhage, glomerular hemorrhage and necrosis, interstitial fibrous tissue proliferation, decreased urinary creatinine clearance, and increased urinary protein level. In addition, studies have found that PPM1K is essential for cell survival, apoptosis and metabolism. However, no study has confirmed that PPM1K can inhibit renal cell apoptosis. In this study, PPM1K was overexpressed in human kidney-2 cells (HK-2), and the biological process of differentially expressed genes and its effect on apoptosis were comprehensively screened by RNA sequencing (RNA-seq). Through sequencing analysis, we found that there were 796 differentially expressed genes in human renal tubular epithelial cells transfected with PPM1K gene, of which 553 were down-regulated and 243 were up-regulated. Enrichment analysis found that differentially expressed genes may play an important role in amino acid metabolism and biosynthesis. In the GO analysis functional pathway list, we also found that multiple genes can be enriched in apoptosis related pathways, such as G0S2, GADD45A, TRIB3, VEGFA, NUPR1 and other up-regulated genes, and IL-6, MAGED1, CCL2, TP53INP1 and other down-regulated genes. Then we verified these differentially expressed genes by RT-PCR, and found that only the RT-PCR results of G0S2, VEGFA and NUPR1 were consistent with the transcriptome sequencing results. We believe that G0S2, VEGFA, NUPR1 and other genes may participate in the apoptosis process of HK-2 cells induced by PPM1K.In conclusion, these findings provide some data support for the study of HK-2 cell apoptosis mechanism, and also provide a scientific theoretical basis for further study of the effect of PPM1K on kidney disease.
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