Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly

Shijie Huang; Nikolay A. Aleksashin; Anna B. Loveland; Dorota Klepacki; Kaspar Reier; Amira Kefi; Teresa Szal; Jaanus Remme; Luc Jaeger; Nora Vázquez-Laslop; Andrei A. Korostelev; Alexander S. Mankin

doi:10.1038/s41467-020-16694-8

Nature Communications (Jun 2020)

Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly

Shijie Huang,
Nikolay A. Aleksashin,
Anna B. Loveland,
Dorota Klepacki,
Kaspar Reier,
Amira Kefi,
Teresa Szal,
Jaanus Remme,
Luc Jaeger,
Nora Vázquez-Laslop,
Andrei A. Korostelev,
Alexander S. Mankin

Affiliations

Shijie Huang: Center for Biomolecular Sciences, University of Illinois at Chicago
Nikolay A. Aleksashin: Center for Biomolecular Sciences, University of Illinois at Chicago
Anna B. Loveland: RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School
Dorota Klepacki: Center for Biomolecular Sciences, University of Illinois at Chicago
Kaspar Reier: Institute of Molecular and Cellular Biology, University of Tartu
Amira Kefi: Center for Biomolecular Sciences, University of Illinois at Chicago
Teresa Szal: Center for Biomolecular Sciences, University of Illinois at Chicago
Jaanus Remme: Institute of Molecular and Cellular Biology, University of Tartu
Luc Jaeger: Chemistry and Biochemistry Department, University of California
Nora Vázquez-Laslop: Center for Biomolecular Sciences, University of Illinois at Chicago
Andrei A. Korostelev: RNA Therapeutics Institute, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School
Alexander S. Mankin: Center for Biomolecular Sciences, University of Illinois at Chicago

DOI: https://doi.org/10.1038/s41467-020-16694-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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Ribosomes of all organisms have retained 5S rRNA as an autonomous rRNA species. Here the authors engineer a bacterial strain with ribosomes that do not have free 5S rRNA, and carry structural analyses that suggest the evolutionary preservation of 5S rRNA as an independent molecule is based on its role in the dynamic process of ribosome biogenesis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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