Gut Microbes (Dec 2024)

Clostridium sporogenes-derived metabolites protect mice against colonic inflammation

  • Felix F. Krause,
  • Kira I. Mangold,
  • Anna-Lena Ruppert,
  • Hanna Leister,
  • Anne Hellhund-Zingel,
  • Aleksandra Lopez Krol,
  • Jelena Pesek,
  • Bernhard Watzer,
  • Sarah Winterberg,
  • Hartmann Raifer,
  • Kai Binder,
  • Ralf Kinscherf,
  • Alesia Walker,
  • Wolfgang A. Nockher,
  • R. Verena Taudte,
  • Wilhelm Bertrams,
  • Bernd Schmeck,
  • Anja A. Kühl,
  • Britta Siegmund,
  • Rossana Romero,
  • Maik Luu,
  • Stephan Göttig,
  • Isabelle Bekeredjian-Ding,
  • Ulrich Steinhoff,
  • Burkhard Schütz,
  • Alexander Visekruna

DOI
https://doi.org/10.1080/19490976.2024.2412669
Journal volume & issue
Vol. 16, no. 1

Abstract

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Gut microbiota-derived metabolites play a pivotal role in the maintenance of intestinal immune homeostasis. Here, we demonstrate that the human commensal Clostridium sporogenes possesses a specific metabolic fingerprint, consisting predominantly of the tryptophan catabolite indole-3-propionic acid (IPA), the branched-chain acids (BCFAs) isobutyrate and isovalerate and the short-chain fatty acids (SCFAs) acetate and propionate. Mono-colonization of germ-free mice with C. sporogenes (CS mice) affected colonic mucosal immune cell phenotypes, including up-regulation of Il22 gene expression, and increased abundance of transcriptionally active colonic tuft cells and Foxp3+ regulatory T cells (Tregs). In DSS-induced colitis, conventional mice suffered severe inflammation accompanied by loss of colonic crypts. These symptoms were absent in CS mice. In conventional, but not CS mice, bulk RNAseq analysis of the colon revealed an increase in inflammatory and Th17-related gene signatures. C. sporogenes-derived IPA reduced IL-17A protein expression by suppressing mTOR activity and by altering ribosome-related pathways in Th17 cells. Additionally, BCFAs and SCFAs generated by C. sporogenes enhanced the activity of Tregs and increased the production of IL-22, which led to protection from colitis. Collectively, we identified C. sporogenes as a therapeutically relevant probiotic bacterium that might be employed in patients with inflammatory bowel disease (IBD).

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