Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry

Zhongliang Zhang; Qiang Li; Qiufen Li; Simiao Du; Yongquan Zhou; Chunming Lv; Yan Zhao; Yongjun Wang; Ning Zhang

doi:10.1016/j.apsb.2014.05.003

Acta Pharmaceutica Sinica B (Oct 2014)

Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry

Zhongliang Zhang,
Qiang Li,
Qiufen Li,
Simiao Du,
Yongquan Zhou,
Chunming Lv,
Yan Zhao,
Yongjun Wang,
Ning Zhang

Affiliations

Zhongliang Zhang: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Qiang Li: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Qiufen Li: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Simiao Du: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Yongquan Zhou: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Chunming Lv: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Yan Zhao: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Yongjun Wang: Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
Ning Zhang: Experimental Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

DOI: https://doi.org/10.1016/j.apsb.2014.05.003
Journal volume & issue: Vol. 4, no. 5
pp. 384 – 393

Abstract

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Qi She Pill (QSP) is a traditional Chinese medicine (TCM) prescription that has been used in treating cervical spondylosis radiculopathy for many years. In this study, a simple and sensitive method using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP. We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol. The correlation coefficients of all calibration curves were greater than 0.99. Recoveries measured at three concentration levels varied from 95.43% to 102.35%. Relative standard deviations of intra- and inter-day precisions were less than 4.45%. After successfully validating our method, we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components.

Published in Acta Pharmaceutica Sinica B

ISSN: 2211-3835 (Print); 2211-3843 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/acta-pharmaceutica-sinica-b

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