Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise

Abstract

Summary: The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mice and the region-specific sampling of blood from the liver and hindlimb. This procedure is particularly relevant for the study of tissue-specific metabolism in response to exercise.For complete details on the use and execution of this protocol, please refer to Sato et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Behavior
• Health Sciences
• Metabolism
• Model Organisms