Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

Sunisa Yoodee; Chadanat Noonin; Kanyarat Sueksakit; Rattiyaporn Kanlaya; Sakdithep Chaiyarit; Paleerath Peerapen; Visith Thongboonkerd

doi:10.1038/s42003-021-02479-2

Communications Biology (Aug 2021)

Effects of secretome derived from macrophages exposed to calcium oxalate crystals on renal fibroblast activation

Sunisa Yoodee,
Chadanat Noonin,
Kanyarat Sueksakit,
Rattiyaporn Kanlaya,
Sakdithep Chaiyarit,
Paleerath Peerapen,
Visith Thongboonkerd

Affiliations

Sunisa Yoodee: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University
Chadanat Noonin: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University
Kanyarat Sueksakit: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University
Rattiyaporn Kanlaya: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University
Sakdithep Chaiyarit: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University
Paleerath Peerapen: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University
Visith Thongboonkerd: Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University

DOI: https://doi.org/10.1038/s42003-021-02479-2
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 11

Abstract

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Yoodee et al use label-free quantitative proteomics (nanoLC-ESI-LTQ-Orbitrap MS/MS) to identify 23 significantly altered secreted proteins from calcium oxalate monohydrate (COM)-exposed macrophages secretome. They demonstrate that proteins secreted from macrophages exposed to COM crystals induce renal fibroblast activation and may play important roles in renal fibrogenesis in kidney stone disease.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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