Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform

Vladimir Riazanski; Gerardo Mauleon; Kilean Lucas; Samuel Walker; Adriana M. Zimnicka; James L. McGrath; Deborah J. Nelson

doi:10.1038/s42003-021-02965-7

Communications Biology (Jan 2022)

Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform

Vladimir Riazanski,
Gerardo Mauleon,
Kilean Lucas,
Samuel Walker,
Adriana M. Zimnicka,
James L. McGrath,
Deborah J. Nelson

Affiliations

Vladimir Riazanski: Department of Pharmacological and Physiological Sciences, The University of Chicago
Gerardo Mauleon: Department of Pharmacological and Physiological Sciences, The University of Chicago
Kilean Lucas: Department of Biomedical Engineering, University of Rochester
Samuel Walker: Department of Biomedical Engineering, University of Rochester
Adriana M. Zimnicka: Department of Pharmacological and Physiological Sciences, The University of Chicago
James L. McGrath: Department of Biomedical Engineering, University of Rochester
Deborah J. Nelson: Department of Pharmacological and Physiological Sciences, The University of Chicago

DOI: https://doi.org/10.1038/s42003-021-02965-7
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 13

Abstract

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Riazanski et al describe a platform to capture extracellular vesicles (EVs) using a nanoporous silicon nitride membrane, investigate the expression of NHE1 protein on the surface of EVs and monitor the transport of Na+ and H+ at the single EV level. The authors report a mechanistic function of the proteins found in EVs and specifically identify NHE1 on a single EV, where it maintains pH neutrality within single vesicles.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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