Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

Jan Homolak; Ivan Kodvanj; Ana Babic Perhoc; Davor Virag; Ana Knezovic; Jelena Osmanovic Barilar; Peter Riederer; Melita Salkovic-Petrisic

MethodsX (Jan 2022)

Nitrocellulose redox permanganometry: A simple method for reductive capacity assessment

Jan Homolak,
Ivan Kodvanj,
Ana Babic Perhoc,
Davor Virag,
Ana Knezovic,
Jelena Osmanovic Barilar,
Peter Riederer,
Melita Salkovic-Petrisic

Affiliations

Jan Homolak: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia; Corresponding author at: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia.
Ivan Kodvanj: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Ana Babic Perhoc: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Davor Virag: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Ana Knezovic: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Jelena Osmanovic Barilar: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Peter Riederer: Center of Mental Health, Department of Psychiatry, Psychosomatics and Psychotherapy, University Hospital Würzburg, Würzburg, Germany; Department and Research Unit of Psychiatry, Institute of Clinical Research, University of Southern Denmark, Odense, Denmark
Melita Salkovic-Petrisic: Department of Pharmacology, University of Zagreb School of Medicine, Zagreb, Croatia; Research Centre of Excellence, Institute of Fundamental Clinical and Translational Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia

Journal volume & issue: Vol. 9
p. 101611

Abstract

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We propose a rapid, simple, and robust method for measurement of the reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations. • NRP is a rapid, cost-effective, and simple method for reductive capacity assessment • NRP is compatible with a high-throughput screening of solid and liquid biological samples • HistoNRP exploits passive diffusion slice print blotting for reductive capacity spatial analysis

Nitrocellulose Redox Permanganometry (NRP)

Published in MethodsX

ISSN: 2215-0161 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science
Website: http://www.journals.elsevier.com/methodsx/

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