Dietary acidic calcium sulfate enhances growth, digestive enzyme activities, intestinal histology and resistance against Aeromonas hydrophila in juvenile largemouth bass, Micropterus salmoides

He Huang; Yi Zhuang; Xiang-Shan Tang; Nu-An Wang; Kun Yuan; Guo-Fang Zhong

Aquaculture Reports (Apr 2023)

Dietary acidic calcium sulfate enhances growth, digestive enzyme activities, intestinal histology and resistance against Aeromonas hydrophila in juvenile largemouth bass, Micropterus salmoides

He Huang,
Yi Zhuang,
Xiang-Shan Tang,
Nu-An Wang,
Kun Yuan,
Guo-Fang Zhong

Affiliations

He Huang: National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Yi Zhuang: National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Xiang-Shan Tang: Zhejiang Hangzhou Tiao wang Biological Technology Co., Ltd., Hangzhou 310015,China
Nu-An Wang: South China Agricultural University, Guangzhou 510640, China
Kun Yuan: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
Guo-Fang Zhong: National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai 201306, China; Corresponding author at: National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.

Journal volume & issue: Vol. 29
p. 101467

Abstract

Read online

This study was conducted to investigate the effects of dietary supplementation with acidic calcium sulfate on growth, intestinal histology, and disease resistance of juvenile largemouth bass. Fish (19.88 ± 0.03 g) were fed on diets supplemented with 0 % (control), 1% acidic calcium sulfate (ACS1), 2 % acidic calcium sulfate (ACS2), 3 % acidic calcium sulfate (ACS3) and 0.03 % florfenicol (F) for 56 days. The results showed that the supplementation of 1 % acidic calcium sulfate to the diet significantly enhanced the growth performance (P < 0.05). Serum alanine aminotransferase activity in ACS2 and ACS3 groups was significantly higher than that in the control and ACS1 groups (P < 0.05). The serum alkaline phosphatase activity in ACS1 and F groups was significantly higher than that in the control and ACS3 groups (P < 0.05). The activity of serum superoxide dismutase in the control, ACS2 and ACS3 groups was significantly lower than that in ACS1 group (P < 0.05). Intestinal trypsin, amylase, and lipase activities increased significantly with increasing acidic calcium sulfate when compared to the control. (P < 0.05). The intestinal histology features showed that the villi height and width of ACS1 and ACS2 groups were significantly increased compared to that of the control (P < 0.05). The results of TOR pathway related genes in the liver showed that the expressions of TOR and ribosomal protein S6 of ACS1 group were significantly higher than the control, while the expression of 4E-BP1 was significantly decreased (P < 0.05). When challenged with Aeromonas hydrophila, the survival rate of the control was significantly lower than that of F, ACS1, and ACS2 groups (P < 0.05). In summary, the optimum amount of acidic calcium sulfate for this experiment is 1 %. 1 % acidic calcium sulfate can effectively improve the growth performance and disease resistance of juvenile largemouth bass.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

About the journal

Abstract

Keywords