Anticancer effect of superoxide dismutase on canine mammary gland tumour in vitro

Abstract

Abstract Background Reactive oxygen species (ROS) have been shown to promote tumour growth and metastasis in human cell lines. The superoxide anion (•O2−) is produced during ROS formation and is involved in tumour cell signalling. Objectives Superoxide dismutase (SOD) has been applied to canine mammary gland tumours to investigate its antitumour effects in vitro. Methods Cell proliferation, cell cycle cell migration assays, reverse transcription‐quantitative polymerase chain reaction, and western blot analysis were performed to determine the effects of SOD on canine mammary tumour cell line. Results SOD treatment resulted in anti‐proliferative effects and mediated cell cycle arrest in the canine mammary gland tumour cell lines (CIPp and CIPm). It also downregulated the expression of N‐cadherin and Vimentin. Conclusions The results confirmed that SOD inhibits tumour cell proliferation and migration, thus supporting the potential applications of SOD as a chemotherapeutic agent for canine mammary gland tumours.

Keywords

• anticancer effect
• dog
• mammary gland tumour
• superoxide dismutase