Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter

Guangjie Cui; Yunbo Liu; Di Zu; Xintao Zhao; Zhijia Zhang; Do Young Kim; Pramith Senaratne; Aaron Fox; David Sept; Younggeun Park; Somin Eunice Lee

doi:10.1038/s41467-023-39624-w

Nature Communications (Jul 2023)

Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter

Guangjie Cui,
Yunbo Liu,
Di Zu,
Xintao Zhao,
Zhijia Zhang,
Do Young Kim,
Pramith Senaratne,
Aaron Fox,
David Sept,
Younggeun Park,
Somin Eunice Lee

Affiliations

Guangjie Cui: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan
Yunbo Liu: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan
Di Zu: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan
Xintao Zhao: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan
Zhijia Zhang: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan
Do Young Kim: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan
Pramith Senaratne: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan
Aaron Fox: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan
David Sept: Department of Biomedical Engineering, University of Michigan
Younggeun Park: Department of Mechanical Engineering, University of Michigan
Somin Eunice Lee: Department of Electrical & Computer Engineering, Biomedical Engineering, Applied Physics, Biointerfaces Institute, Macromolecular Science & Engineering, University of Michigan

DOI: https://doi.org/10.1038/s41467-023-39624-w
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 10

Abstract

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Abstract Fundamental to all living organisms and living soft matter are emergent processes in which the reorganization of individual constituents at the nanoscale drives group-level movements and shape changes at the macroscale over time. However, light-induced degradation of fluorophores, photobleaching, is a significant problem in extended bioimaging in life science. Here, we report opening a long-time investigation window by nonbleaching phase intensity nanoscope: PINE. We accomplish phase-intensity separation such that nanoprobe distributions are distinguished by an integrated phase-intensity multilayer thin film (polyvinyl alcohol/liquid crystal). We overcame a physical limit to resolve sub-10 nm cellular architectures, and achieve the first dynamic imaging of nanoscopic reorganization over 250 h using PINE. We discover nanoscopic rearrangements synchronized with the emergence of group-level movements and shape changes at the macroscale according to a set of interaction rules with importance in cellular and soft matter reorganization, self-organization, and pattern formation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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