Nature Communications (Oct 2022)

Genome-wide functional screening of drug-resistance genes in Plasmodium falciparum

  • Shiroh Iwanaga,
  • Rie Kubota,
  • Tsubasa Nishi,
  • Sumalee Kamchonwongpaisan,
  • Somdet Srichairatanakool,
  • Naoaki Shinzawa,
  • Din Syafruddin,
  • Masao Yuda,
  • Chairat Uthaipibull

DOI
https://doi.org/10.1038/s41467-022-33804-w
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 12

Abstract

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Plasmodium falciparum malaria is treated using artemisinin combination therapy (ACT), in which artemisinin is supplied along with partner drugs such as mefloquine, piperaquine, and lumefantrine. However, resistance has been reported in endemic regions. To identifying new effector genes involved in resistance, Iwanaga et al. develop a large scale transgenic screen with genomic libraries of resistance strains. Using this approach they provide evidence that transcriptional upregulation of pfmdr7 contributes to mefloquine resistance in a clinical isolate.