LncRNA UNC5B-AS1 targeting miR-339-5p regulates the proliferation and apoptosis of human lung adenocarcinoma cell line A549

Abstract

Objective To study the effect of lncRNA UNC5B-AS1 targeting miR-339-5p on the proliferation and apoptosis of lung cancer cells and its molecular mechanism. Methods The human lung adenocarcinoma cell line A549 was cultured in vitro, and si-NC, si-UNC5B-AS1, miR-NC, miR-339-5p mimics, si-UNC5B-AS1 and anti-miR-NC, si-UNC5B-AS1 and anti-miR-339-5p were transfected into A549 cells respectively. Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of UNC5B-AS1 and miR-339-5p in lung cancer tissues and in adjacent tissues; Cell counting kit (CCK-8) was used to detecte cell proliferation; The targeted regulation relationship between UNC5B-AS1 and miR-339-5p was examined by dual luciferase reporter gene experiment; Western blot was used to detect the expression of cyclin D1 (cyclin D1), p21 protein lymphoma-2(Bcl-2), and B lymphoma-2 related protein (Bax). Results Compared with adjacent tissues, the expression level of UNC5B-AS1 in lung cancer tissue was significantly increased (P＜0.05), and the expression level of miR-339-5p was significantly decreased(P＜0.05). Compared with the si-NC group, the cell viability of the si-UNC5B-AS1 group was significantly reduced (P＜0.05), the apoptosis rate was significantly higher(P＜0.05). Compared with the miR-NC group, the cell viability of the miR-339-5p group was significantly reduced (P＜0.05), and the apoptosis rate was significantly increased(P＜0.05). Compared with the (si-UNC5B-AS1+anti-miR-NC)group, the cell viability of the (si-UNC5B-AS1+anti-miR-339-5p) group was significantly increased (P＜0.05); the apoptosis rate was significantly reduced (P＜0.05). Conclusions Interference with UNC5B-AS1 promotes apoptosis and inhibits cell proliferation of human lung adenocarcinoma cancer cell line A549 by up-regulating the expression of miR-339-5p.

Keywords

• lncrna unc5b-as1|mir-339-5p|lung cancer|proliferation|apoptosis