Micropropagation of <i>Dieffenbachia picta</i>

Abstract

Dieffenbachia picta is an ornamental aroid of foliage with high commercial value. With the purpose of achieving a micropropagation protocol, experiments were developed in the establishment stage, multiplication and adaptation ex vitro. It was determined that the inactive axillary buds are adapted as explant according to their percentage of contamination, survival and growth. The establishment of these buds was effective to the 120 days with combinations of BAP (0.5 and 1.0 mg.l-1) and AIA (0.1 and 1.9 mg.l-1). The multiplication was obtained in the second reculture with high citoquinina level (16. mg.l-1 of 2iP) and tipped of the explants. The shoots took root in the phase II and they adapted appropriately to the conditions ex vitro. Key words: araceae, axilary buds, cytokinins, multiplication, ornamental