The Analysis of the Foxp2 and ASCL1 Expression in Saffron-Treated Mice

Abstract

Background & Objective: The aim of this study was to investigate the effect of saffron extract on the embryo development of mice. Materials & Methods: Pregnant NMRI mice were randomized into control and treatment groups at 25, 50, and 100 mg/kg saffron doses. Saffron extract was administered to mice by gavage on days 7-12 of pregnancy. On the 17th day of pregnancy, the embryos were removed from the uterus and their weight and height measured. Moreover, their brain tissue has been evaluated histologically. Subsequently, the expression of the Foxp2 and Ascl1 genes in the brain tissue was assessed using the real-time PCR method. Results: All embryos were aborted in mothers that received 100 mg/kg of saffron. At dose 50 mg/kg, only embryos of a mother reached the end of pregnancy. Embryos treated with 25 mg/kg saffron were significantly heavier than controls (P<0.05). Furthermore, the tail length was significantly shorter (P<0.05). Histological findings showed that there was no difference between the control and treated groups, and the brain tissue was well developed. Foxp2 and Ascl1 genes were significantly overexpressed in both the 25 and 50 mg/kg treatment groups compared to the control group (P<0.05). Conclusion: The results of this study showed that saffron extract can have significant effects in low concentrations (25 mg/kg) on the development of the mouse embryos as well as the expression of Foxp2 and Ascl1 genes.

Keywords

• saffron
• embryo
• mouse
• foxp2 gene
• ascl1 gene