Chromatography methods in investigation of lipophilicity of the biological active substances

Abstract

This paper presents the review of the methods used in research of the biological active substances hydrophobicity, a very important property. The biological activity of some substances depends on their pharmacokinetics and pharmacodynamics. These processes depend on the molecule's capability to interact with two different media: aqueous (cells interior) and non-aqueous (cells membrane), or on the molecule lipophilicity. Today, great attention is given to investigation and systematic determination of drugs lipophilicity. In these researches chromatography methods have an important role.

Keywords

• lipophilicity
• hydrophobicity
• shake flask method
• high performance liquid chromatography
• thin layer chromatography