Type IV pilus retraction is required for Neisseria musculi colonization and persistence in a natural mouse model of infection

Abstract

ABSTRACTRetraction of the pathogenic Neisseria Type IV pilus (Tfp) requires PilT, the primary retraction motor, and its paralogs PilU and PilT2. The importance of Tfp retraction for natural infection is unknown. Using our natural animal model of Neisseria-host interaction, we have examined the role of these proteins in the ability of commensal Neisseria musculi (Nmus) to colonize and persist in its native host, the mouse. We report that Nmus ΔpilT cannot colonize mice; ΔpilU and ΔpilT2 can colonize and persist in mice, but in lower numbers. Microcolonies formed by ΔpilT, ΔpilTU, and pilTL201C, expressing PilT with a point mutation in its ATP hydrolysis domain, are sensitive to removal by fluid shear forces. Thus, PilT promotes Nmus colonization while PilU and PilT2 influence persistence. We present a non-exclusive model for how these Tfp retraction motor proteins contribute to Nmus persistent colonization. Our findings have implications for the roles of these motor proteins in mediating interactions of human-adapted pathogenic and commensal Neisseria with their human host.IMPORTANCEWe describe the importance of Type IV pilus retraction to colonization and persistence by a mouse commensal Neisseria, N. musculi, in its native host. Our findings have implications for the role of Tfp retraction in mediating interactions of human-adapted pathogenic and commensal Neisseria with their human host due to the relatedness of these species.

Keywords

• Type IV pilus retraction
• PilT
• PilU
• PilT2
• Neisseria musculi
• Neisseria gonorrhoeae