Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva

Ekaterina Kondrateva; Olga Grigorieva; Elizaveta Kurshakova; Irina Panchuk; Victoria Pozhitnova; Ekaterina Voronina; Vyacheslav Tabakov; Maria Orlova; Alexander Lavrov; Svetlana Smirnikhina; Sergey Kutsev

Stem Cell Research (Aug 2023)

Generation of induced pluripotent stem cell line (RCMGi009-A) from urine cells of patient with fibrodysplasia ossificans progressiva

Ekaterina Kondrateva,
Olga Grigorieva,
Elizaveta Kurshakova,
Irina Panchuk,
Victoria Pozhitnova,
Ekaterina Voronina,
Vyacheslav Tabakov,
Maria Orlova,
Alexander Lavrov,
Svetlana Smirnikhina,
Sergey Kutsev

Affiliations

Ekaterina Kondrateva: Corresponding author.; Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Olga Grigorieva: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Elizaveta Kurshakova: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Irina Panchuk: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Victoria Pozhitnova: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Ekaterina Voronina: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Vyacheslav Tabakov: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Maria Orlova: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Alexander Lavrov: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Svetlana Smirnikhina: Research Centre for Medical Genetics, Moscow 115522, Russian Federation
Sergey Kutsev: Research Centre for Medical Genetics, Moscow 115522, Russian Federation

Journal volume & issue: Vol. 70
p. 103133

Abstract

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Urine cells obtained from a 14-year-old man with genetically proven (ACVR1: c.6176G > A) and clinically manifested fibrodysplasia ossificans progressiva were successfully transformed into induced pluripotent stem cells by using Sendai virus-based reprogramming vectors including the four Yamanaka factors such as OCT3/4, SOX2, KLF4, and c-MYC. These iPSCs expressed pluripotency markers, exhibited the potential to differentiate into three germ layers in spontaneous differentiation assay and had a normal karyotype. The iPSC line may provide a model for development of a personalized treatment including genome editing and drug screening, may be used for disease modelling, cell differentiation and pharmacological investigations..

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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