Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich <i>Portulaca grandiflora</i> Hook. Extracts

Aneta Spórna-Kucab; Anna Tekieli; Agnieszka Grzegorczyk; Łukasz Świątek; Barbara Rajtar; Krystyna Skalicka-Woźniak; Karolina Starzak; Boris Nemzer; Zbigniew Pietrzkowski; Sławomir Wybraniec

doi:10.3390/antiox11091654

Antioxidants (Aug 2022)

Metabolite Profiling Analysis and the Correlation with Biological Activity of Betalain-Rich <i>Portulaca grandiflora</i> Hook. Extracts

Aneta Spórna-Kucab,
Anna Tekieli,
Agnieszka Grzegorczyk,
Łukasz Świątek,
Barbara Rajtar,
Krystyna Skalicka-Woźniak,
Karolina Starzak,
Boris Nemzer,
Zbigniew Pietrzkowski,
Sławomir Wybraniec

Affiliations

Aneta Spórna-Kucab: Department of Chemical Technology and Environmental Analysis, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, Poland
Anna Tekieli: Department of Chemical Technology and Environmental Analysis, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, Poland
Agnieszka Grzegorczyk: Chair and Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodźki Str, 20-093 Lublin, Poland
Łukasz Świątek: Department of Virology with SARS Laboratory, Medical University of Lublin, 1 Chodźki Str, 20-093 Lublin, Poland
Barbara Rajtar: Department of Virology with SARS Laboratory, Medical University of Lublin, 1 Chodźki Str, 20-093 Lublin, Poland
Krystyna Skalicka-Woźniak: Department of Natural Products Chemistry, Medical University of Lublin, 1 Chodźki Str, 20-093 Lublin, Poland
Karolina Starzak: Department of Chemical Technology and Environmental Analysis, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, Poland
Boris Nemzer: Research and Analytical Center, VDF FutureCeuticals, Inc., 2692 N. State Rt. 1-17, Momence, IL 60954, USA
Zbigniew Pietrzkowski: Discovery Research Lab, VDF FutureCeuticals, Inc., 23 Peters Canyon Rd., Irvine, CA 92606, USA
Sławomir Wybraniec: Department of Chemical Technology and Environmental Analysis, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, Poland

DOI: https://doi.org/10.3390/antiox11091654
Journal volume & issue: Vol. 11, no. 9
p. 1654

Abstract

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The aim of the study was to evaluate the possible correlation between the bioactivity and the phytochemical profile of four betalain-rich extracts from Portulaca grandiflora Hook. The HPLC-DAD-ESI-MS analysis indicated the presence of 19 betaxanthins and two betacyanins. The highest concentrations of betaxanthins (982 mg/100 g DE) and betacyanins (650 mg/100 g DE) were noticed in orange and purple flowers extracts, respectively. The HPLC-DAD-ESI-HRMS/MS analyses revealed the presence of a total of 71 compounds. Fifteen new betaxanthins and fifty other metabolites were identified for the first time. The antioxidant activity of the studied flower extracts increased in the sequence of yellow P. grandiflora extracts were non-toxic to normal VERO cells. No significant antiviral activity towards Human Herpesvirus type 1 was observed (62 µg/mL). Among the tested varieties, the purple one showed anticancer selectivity towards colon carcinoma cells (RKO).

Published in Antioxidants

ISSN: 2076-3921 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.mdpi.com/journal/antioxidants

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