Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene

L.D. Shuvalova; A.V. Eremeev; A.N. Bogomazova; E.V. Novosadova; E.A. Zerkalenkova; Yu.V. Olshanskaya; E.Y. Fedotova; E.S. Glagoleva; S.N. Illarioshkin; O.S. Lebedeva; M.A. Lagarkova

Stem Cell Research (Apr 2020)

Generation of induced pluripotent stem cell line RCPCMi004-A derived from patient with Parkinson's disease with deletion of the exon 2 in PARK2 gene

L.D. Shuvalova,
A.V. Eremeev,
A.N. Bogomazova,
E.V. Novosadova,
E.A. Zerkalenkova,
Yu.V. Olshanskaya,
E.Y. Fedotova,
E.S. Glagoleva,
S.N. Illarioshkin,
O.S. Lebedeva,
M.A. Lagarkova

Affiliations

L.D. Shuvalova: Federal State Budgetary Institution, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia
A.V. Eremeev: Federal State Budgetary Institution, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia; Corresponding author.
A.N. Bogomazova: Federal State Budgetary Institution, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia
E.V. Novosadova: Russian Academy of Sciences, Institute of Molecular Genetics, Kurchatov Square 2, 123182 Moscow, Russia
E.A. Zerkalenkova: Rogachev Federal Scientific and Clinical Centre of Pediatric Hematology Oncology and Immunology, 1 Samory Mashela str, 117997 Moscow, Russia
Yu.V. Olshanskaya: Rogachev Federal Scientific and Clinical Centre of Pediatric Hematology Oncology and Immunology, 1 Samory Mashela str, 117997 Moscow, Russia
E.Y. Fedotova: Federal Research Centre of Neurology, Russian Academy of Sciences, 80, Volokolamskoye Shosse, 125367 Moscow, Russia
E.S. Glagoleva: Lomonosov Moscow State University, GSP-1, Leninskie Gory, 119991 Moscow, Russia
S.N. Illarioshkin: Federal Research Centre of Neurology, Russian Academy of Sciences, 80, Volokolamskoye Shosse, 125367 Moscow, Russia
O.S. Lebedeva: Federal State Budgetary Institution, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia
M.A. Lagarkova: Federal State Budgetary Institution, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, Russia; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia

Journal volume & issue: Vol. 44

Abstract

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IPSC line RCPCMi004-A was generated from skin fibroblasts collected from a male patient with early onset Parkinson's disease. The patient carries a heterozygous deletion of the exon 2 of PARK2 gene. The reprogramming of fibroblasts was performed with Sendai viruses containing Oct-4, Sox-2, Klf-4 and c-Myc. Pluripotency was confirmed by immunofluorescence, RT-PCR, and formation of embryoid bodies. The RCPCMi004-A cell line carries the same deletion in PARK2 gene. The RCPCMi004-A cell line can be used to model Parkinson's disease in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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