Evaluation the Efficacy of Sodium Valproate and Low Frequency Stimulation During CA1 Hippocampal Kindling on Anxiety-Like Behavior in Adult Male Rat

Abstract

Background and Aim: Emotional disorders are prevalent in many epileptic patients. So, in this research, we have studied the efficacy of two treatment methods of seizure on anxiety-like behavior during kindling in adult male rat. Materials and Methods: 42 male rats were randomly divided into 6 groups: Control, Sham operation, saline-kindled and drug-kindled groups which have received saline or drug 15 minutes before kindling stimulations, and saline-kindled-LFS or drug-kindled-LFS group which have received saline or drug 15 minutes before kindling stimulations and LFS applied after termination of kindling stimulations. Anxiety-like behavior was assessed on the 6th day by using elevated plus maze and open field apparatus. Findings: kindling significantly increased open arms (OAs) entries percentage, OAs exploration percentage, increasing jumping from elevated plus maze (p<0.001) and rearing frequency in open field apparatus (p<0.05) compared to the sham group. Sodium valproate increased OAs entries percentage and OAs exploration percentage in drug-kindled group compared to sham group (p<0.001). But, there wasn’t any significant difference in jumping from elevated plus maze and rearing in open field compared to sham group. Also, there was no significant change in these parameters in Saline-kindled-LFS, drug-kindled and drug–kindled-LFS groups. Conclusion: Sodium valproate and LFS, as two therapies controlling epilepsy, decrease anxiety induced by kindling stimulation.

Keywords

• Sodium valproate
• Low frequency stimulation
• Kindling
• Anxiety