PARTICIPATION OF REDOX SIGNALIZATION IN NITRIC OXIDE-, CARBON MONOXIDE- AND HUDROGEN SULFIDE-MEDIATED REGULATION OF APOPTOSIS AND CELL CYCLE

Abstract

The study has demonstrated that proapoptic concentrations of donors of NO (100 mmol SNP and 100 µmol NOC-5), H2S (10 mmol NaHS), and CO (50 µmol CORM-2) gases caused an increase in the intracellular level of active forms of oxygen in Jurkat cells. As this took place, the activation of redox-dependent transcription factor р53 was observed as Jurkat cells were exposed to 100 mmol SNP and 10 mmol NaHS. In the case of 100 µmol NOC-5 and 50 µmol CORM-2, an increase of р53 was not observed, but the expression of target gens of this transcription factor р21 (under the effect of NO and СО) and bax (under the effect of NO) increased. The antiproliferative concentration of hydrogen sulfide donor (50 µmol ) did not cause an increase in the intracellular production of active forms of oxygen and the activation of redox-dependent signal mechanisms.

Keywords

• активные формы кислорода
• оксид азота
• сульфид водорода
• монооксид углерода