Nature Communications (Nov 2018)

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

  • Panchali Goswami,
  • Ferdos Abid Ali,
  • Max E. Douglas,
  • Julia Locke,
  • Andrew Purkiss,
  • Agnieszka Janska,
  • Patrik Eickhoff,
  • Anne Early,
  • Andrea Nans,
  • Alan M. C. Cheung,
  • John F. X. Diffley,
  • Alessandro Costa

DOI
https://doi.org/10.1038/s41467-018-07417-1
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 13

Abstract

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Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase, which requires the leading-strand polymerase Pol ɛ. Here the authors present a structural analysis of a CMG Pol ɛ on a DNA fork, providing insight on the steps leading productive helicase engagement to the DNA junction.