Visualization of invisible cell-death sign by electric-double-layer modulation

Jun'ya Tsutsumi; Jun Kurosu; Kaname Kanai; Kingo Uchida; Kana Morishita; Kimio Sumaru

Biosensors and Bioelectronics: X (Sep 2023)

Visualization of invisible cell-death sign by electric-double-layer modulation

Jun'ya Tsutsumi,
Jun Kurosu,
Kaname Kanai,
Kingo Uchida,
Kana Morishita,
Kimio Sumaru

Affiliations

Jun'ya Tsutsumi: Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan; Corresponding author. Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
Jun Kurosu: Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan
Kaname Kanai: Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan
Kingo Uchida: Department of Materials Chemistry, Faculty of Advanced Science and Technology, Ryukoku University, Seta, Otsu, Shiga, 520-2194, Japan
Kana Morishita: Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan
Kimio Sumaru: Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan

Journal volume & issue: Vol. 14
p. 100390

Abstract

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Leakage and self-spreading of a cytoplasmic lipid droplet in association with cell death, which was totally invisible in a conventional optical microscope, was discovered by using the recently developed electric-double-layer modulation imaging (EDLMI). The method allowed real-time imaging of the leaked lipid droplet with the thickness of a few nanometers. We demonstrate that the discovered phenomenon is useful as a new cellular sign for diagnosis of cell death.

Published in Biosensors and Bioelectronics: X

ISSN: 2590-1370 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/biosensors-and-bioelectronics-x

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