Nature Communications (Apr 2020)
Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses
- Karol Nass,
- Alexander Gorel,
- Malik M. Abdullah,
- Andrew V. Martin,
- Marco Kloos,
- Agostino Marinelli,
- Andrew Aquila,
- Thomas R. M. Barends,
- Franz-Josef Decker,
- R. Bruce Doak,
- Lutz Foucar,
- Elisabeth Hartmann,
- Mario Hilpert,
- Mark S. Hunter,
- Zoltan Jurek,
- Jason E. Koglin,
- Alexander Kozlov,
- Alberto A. Lutman,
- Gabriela Nass Kovacs,
- Christopher M. Roome,
- Robert L. Shoeman,
- Robin Santra,
- Harry M. Quiney,
- Beata Ziaja,
- Sébastien Boutet,
- Ilme Schlichting
Affiliations
- Karol Nass
- Max-Planck-Institut für Medizinische Forschung
- Alexander Gorel
- Max-Planck-Institut für Medizinische Forschung
- Malik M. Abdullah
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Andrew V. Martin
- School of Science, RMIT University
- Marco Kloos
- Max-Planck-Institut für Medizinische Forschung
- Agostino Marinelli
- SLAC National Accelerator Laboratory
- Andrew Aquila
- SLAC National Accelerator Laboratory
- Thomas R. M. Barends
- Max-Planck-Institut für Medizinische Forschung
- Franz-Josef Decker
- SLAC National Accelerator Laboratory
- R. Bruce Doak
- Max-Planck-Institut für Medizinische Forschung
- Lutz Foucar
- Max-Planck-Institut für Medizinische Forschung
- Elisabeth Hartmann
- Max-Planck-Institut für Medizinische Forschung
- Mario Hilpert
- Max-Planck-Institut für Medizinische Forschung
- Mark S. Hunter
- SLAC National Accelerator Laboratory
- Zoltan Jurek
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Jason E. Koglin
- SLAC National Accelerator Laboratory
- Alexander Kozlov
- ARC Centre of Excellence for Advanced Molecular Imaging, School of Physics, The University of Melbourne
- Alberto A. Lutman
- SLAC National Accelerator Laboratory
- Gabriela Nass Kovacs
- Max-Planck-Institut für Medizinische Forschung
- Christopher M. Roome
- Max-Planck-Institut für Medizinische Forschung
- Robert L. Shoeman
- Max-Planck-Institut für Medizinische Forschung
- Robin Santra
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Harry M. Quiney
- ARC Centre of Excellence for Advanced Molecular Imaging, School of Physics, The University of Melbourne
- Beata Ziaja
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Sébastien Boutet
- SLAC National Accelerator Laboratory
- Ilme Schlichting
- Max-Planck-Institut für Medizinische Forschung
- DOI
- https://doi.org/10.1038/s41467-020-15610-4
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 9
Abstract
The local X-ray-induced dynamics that occur in protein crystals during serial femtosecond crystallography (SFX) measurements at XFELs are not well understood. Here the authors performed a time-resolved X-ray pump X-ray probe SFX experiment, and they observe distinct structural changes in the disulfide bridges and peptide backbone of proteins; complementing theoretical approaches allow them to further characterize the details of the X-ray induced ionization and local structural dynamics.
