EFFICACY OF OVULATION SYNCHRONIZATION REGIMENS ON PREGNANCY RATE TO TIMED ARTIFICIAL INSEMINATION IN ACYCLIC POST-PARTUM NILI-RAVI BUFFALOES

Abstract

The present study compared the two ovulation synchronization treatments combined with a progesterone release device in buffaloes. Ninety acyclic buffaloes were divided into three experimental The blood samples were collected before the start of the trial to determine the reproductive status (anestrous and sub-estrous). In G I and G II, CIDR was inserted for seven days and treated with on the 6th day of protocol. In the protocol, the first estradiol benzoate (EB) and doses were given on day 0 and 2nd EB and doses on day 8 to G I and G II groups, respectively. Whereas, G III served as a control group. The G I and G II groups were observed for heat signs, but fixed time twice AI was done at 48 or 60 hours of CIDR removal, whereas G III was inseminated at detected estrus. The blood samples were also collected prior to the execution of the trial from experimental buffaloes for observing the cyclicity status through serum progesterone level. The results of cyclicity showed 78% anestrous and 22% sub-estrous status in included buffaloes. The effects of synchronization protocols revealed that estrus response was significantly greater in G I and G II groups than in the G III group. The ovulation occurrence, ovulatory follicle szie and pregnancy outcomes were also significantly higher in G I and G II compared to G III. Conclusively, including EB or at the CIDR insertion and removal times effectively enhances estrus, ovulation and pregnancy in sub-estrous and anestrous buffaloes.

Keywords

• acyclic buffaloes
• estrus
• pregnancy
• synchronization protocols