DCNN-4mC: Densely connected neural network based N4-methylcytosine site prediction in multiple species

Abstract

DNA N4-methylcytosine (4mC) being a significant genetic modification holds a dominant role in controlling different biological functions, i.e., DNA replication, DNA repair, gene regulations and gene expression levels. The identification of 4mC sites is important to get insight information regarding different organics mechanisms. However, getting modification prediction from experimental methods is a challenging task due to high expenses and time-consuming techniques. Therefore, computational tools can be a great option for modification identification. Various computational tools are proposed in literature but their generalization and prediction performance require improvement. For this motive, we have proposed a neural network based tool named DCNN-4mC for identifying 4mC sites. The proposed model involves a set of neural network layers with a skip connection which allows to share the shallow features with dense layers. Skip connection have allowed to gather crucial information regarding 4mC sites. In literature, different models are employed on different species hence in many cases different datasets are available for a single species. In this research, we have combined all available datasets to create a single benchmark dataset for every species. To the best of our knowledge, no model in literature is employed on more than six different species. To ensure the generalizability of DCNN-4mC we have used 12 different species for performance evaluation. The DCNN-4mC tool has attained 2% to 14% higher accuracy than state-of-the-art tools on all available datasets of different species. Furthermore, independent test datasets are also engaged and DCNN-4mC have overall yielded high performance in them as well.

Keywords

• Bioinformatics
• 4mC modification
• Computational Biology
• Convolutional neural network
• Deep learning