Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18)

Yael Schlichter Kadosh; Subramani Muthuraman; Khairun Nisaa; Anat Ben-Zvi; Danit Lisa Karsagi Byron; Marilou Shagan; Alexander Brandis; Tevie Mehlman; Jacob Gopas; Rajendran Saravana Kumar; Ariel Kushmaro

Biofilm (Dec 2024)

Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18)

Yael Schlichter Kadosh,
Subramani Muthuraman,
Khairun Nisaa,
Anat Ben-Zvi,
Danit Lisa Karsagi Byron,
Marilou Shagan,
Alexander Brandis,
Tevie Mehlman,
Jacob Gopas,
Rajendran Saravana Kumar,
Ariel Kushmaro

Affiliations

Yael Schlichter Kadosh: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel
Subramani Muthuraman: Chemistry Division, Vellore Institute of Technology, Chennai, India
Khairun Nisaa: Department of Life Science, Ben-Gurion University of the Negev, Beer Sheva, Israel
Anat Ben-Zvi: Department of Life Science, Ben-Gurion University of the Negev, Beer Sheva, Israel
Danit Lisa Karsagi Byron: Department of Civil and Environmental Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel
Marilou Shagan: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel
Alexander Brandis: Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel
Tevie Mehlman: Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel
Jacob Gopas: Department of Microbiology, Immunology and Genetics Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
Rajendran Saravana Kumar: Chemistry Division, Vellore Institute of Technology, Chennai, India; Corresponding author.
Ariel Kushmaro: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel; The Ilse Katz Center for Nanoscale Science and Technology, Ben Gurion University of the Negev, Beer Sheva, Israel; School of Sustainability and Climate Change, Ben Gurion University of the Negev, Beer Sheva, Israel; Corresponding author. Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel.

Journal volume & issue: Vol. 8
p. 100215

Abstract

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Bacterial communication, Quorum Sensing (QS), is a target against virulence and prevention of antibiotic-resistant infections. 16 derivatives of Piperlongumine (PL), an amide alkaloid from Piper longum L., were screened for QS inhibition. PL-18 had the best QSI activity. PL-18 inhibited the lasR-lasI, rhlR-rhlI, and pqs QS systems of Pseudomonas aeruginosa. PL-18 inhibited pyocyanin and rhamnolipids that are QS-controlled virulence elements. Iron is an essential element for pathogenicity, biofilm formation and resilience in harsh environments, its uptake was inhibited by PL-18. Pl-18 significantly reduced the biofilm biovolume including in established biofilms. PL-18-coated silicon tubes significantly inhibited biofilm formation. The transcriptome study of treated P. aeruginosa showed that PL-18 indeed reduced the expression of QS and iron homeostasis related genes, and up regulated sulfur metabolism related genes. Altogether, PL-18 inhibits QS, virulence, iron uptake, and biofilm formation. Thus, PL-18 should be further developed against bacterial infection, antibiotic resistance, and biofilm formation.

Published in Biofilm

ISSN: 2590-2075 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Microbiology
Website: https://www.journals.elsevier.com/biofilm

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