Effects of reused high-fat diet on male rat livers: A histological assessment using H&E stain

Abstract

Fat associated liver conditions may be related to humans consuming foods heated at high temperatures in degraded and reused unstable fats. Consumption of these deep fried foods and the highly oxidized lipids is an emerging public health issue in human populations where the quality of frying fat is neither monitored nor regulated. This study was carried out to histologically assess the effects of reused high fat diets on male rat livers. Eighteen (18) male Sprague Dawley rats were randomly assigned three groups of six rats each and fed a neat 20% high fat diet, a reused 20% high fat diet and a normal rat pellet diet for two months. The animals were sacrificed and their processed livers examined under light microscope. In the study groups’ slides; hepatocellular ballooning, microvesicular steatosis, lobular inflammation, portal inflammation and fibrosis were determined. Gross examination of livers indicated macro nodular cirrhosis. Finally, it was observed that 20% reused high fat diet had caused liver steatosis, in the study animals. The Haematoxylin and eosin light microscopy assessment would contribute to clarify some aspects of liver pathogenesis and public health implications of reused fats.

Keywords

• reused fat
• haematoxylin-eosin
• liver steatosis
• light microscopy
• non alcoholic fat liver disease