Generation of integration-free induced pluripotent stem cell lines from four pediatric ADHD patients

Leoni Grossmann; Cristine Marie Yde Ohki; Christian Döring; Per Hoffmann; Stefan Herms; Anna Maria Werling; Susanne Walitza; Edna Grünblatt

Stem Cell Research (May 2021)

Generation of integration-free induced pluripotent stem cell lines from four pediatric ADHD patients

Leoni Grossmann,
Cristine Marie Yde Ohki,
Christian Döring,
Per Hoffmann,
Stefan Herms,
Anna Maria Werling,
Susanne Walitza,
Edna Grünblatt

Affiliations

Leoni Grossmann: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Cristine Marie Yde Ohki: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Christian Döring: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Per Hoffmann: Human Genomics Research Group, Department of Biomedicine, University of Basel, 4031 Basel, Switzerland; Institute of Human Genetics, University of Bonn, School of Medicine & University Hospital Bonn, 53105 Bonn, Germany
Stefan Herms: Human Genomics Research Group, Department of Biomedicine, University of Basel, 4031 Basel, Switzerland; Institute of Human Genetics, University of Bonn, School of Medicine & University Hospital Bonn, 53105 Bonn, Germany
Anna Maria Werling: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland
Susanne Walitza: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and the ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Switzerland
Edna Grünblatt: Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Zurich, Switzerland; Neuroscience Center Zurich, University of Zurich and the ETH Zurich, Zurich, Switzerland; Zurich Center for Integrative Human Physiology, University of Zurich, Switzerland; Corresponding author at: Department of Child and Adolescent Psychiatry & Psychotherapy, Psychiatric University Hospital Zurich, University of Zurich, Wagistrasse 12, 8952 Schlieren, Switzerland.

Journal volume & issue: Vol. 53
p. 102268

Abstract

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Human induced pluripotent stem cell (iPSC) lines have been derived from four male patients with childhood attention-deficit hyperactivity disorder (ADHD). Children and adolescents between the ages 6 and 18 suffering from ADHD were recruited for this work. Isolated keratinocytes or peripheral blood mononuclear cells from the participants were reprogrammed into iPSCs using non-integrating Sendai virus to deliver the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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